Vasectomy in Mouse Model Using Electrosurgery Machine
نویسنده
چکیده مقاله:
Vasectomy in laboratory animals is a crucial step in the production of surrogate female mice. The surrogate mothers play a key role in successful embryo transfer, most important steps for the production of transgenic animal models, investigation of the preimplantation embryo development, and revitalization of cryopreserved strains. Abdominal and scrotal surgeries are common surgical procedures used in routine veterinary practice to produce vasectomized males. Two different surgical practice, namely electrosurgery and cold surgical practice, have been used as common techniques in operating rooms. Based on current knowledge, there is no published “technical note” as a detailed and step by step guideline to describe vasectomy using an electrosurgery machine (i.e., Bovie machine) in laboratory animal research and breeding facilities.The common problem during the laboratory animal surgery would be animal mortalities as a consequence of profound bleeding. The use of Bovie machine leads to the prevention of profound bleeding during the surgical practice.
منابع مشابه
Utero-tubal embryo transfer and vasectomy in the mouse model.
The transfer of preimplantation embryos to a surrogate female is a required step for the production of genetically modified mice or to study the effects of epigenetic alterations originated during preimplantation development on subsequent fetal development and adult health. The use of an effective and consistent embryo transfer technique is crucial to enhance the generation of genetically modif...
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چکیده ندارد.
Electrosurgery OR Bipolar electrosurgery OR Monopolar electrosurgery OR Monopolar bipolar electrosurgery OR Electrocautery
1 Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, SP, Brazil. 2 Universidade Estadual de Londrina, Londrina, PR, Brazil. 3 Universidade Estadual de Londrina, Programa de Pós-Graduação em Enfermagem, Londrina, PR, Brazil. ABSTRACT Objective: To identify the composition of the smoke produced by electrocautery use during surgery. Method: Integrative review with search for primar...
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BACKGROUND Little evidence supports the use of any one vas occlusion method. Data from a number of studies now suggest that there are differences in effectiveness among different occlusion methods. The main objectives of this study were to estimate the effectiveness of vasectomy by cautery and to describe the trends in sperm counts after cautery vasectomy. Other objectives were to estimate time...
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عنوان ژورنال
دوره 74 شماره 2
صفحات 191- 195
تاریخ انتشار 2019-06-01
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